• br Our group has utilized human fetal

  2018-10-20

  

  Our group has utilized human fetal hearts and a lineage-tracing, Isl1–cre knock-in hESC line as tools to study the developmental programs during human cardiogenesis and to examine the paracrine signaling mechanisms involved in self-renewal (Qyang et al., 2007) and lineage specification (Lui et al.

• Introduction The loss of retinal neurons

  2018-10-20

  

  Introduction The loss of retinal neurons, their connections and supporting glia in ocular degenerative diseases causes permanent blindness, principally because lost photoreceptors and retinal ganglion cells (RGCs) are not replaced and RGC zip inhibitor fail to regenerate (Berry et al., 2008). Clin

• Immunocytochemistry Cells were fixed at room temperature wit

  2018-10-20

  

  Immunocytochemistry. Cells were fixed at room temperature with 4% paraformaldehyde for 10min. Non-specific proteins were blocked by ultra V block (Thermo). The cells were then treated with primary raf kinase overnight at 4°C. Primary antibodies were against TRA-1-60 (1:500, MA1-023, Thermo Fisher),

• To confirm iPS identity and purity we checked embryonic stem

  2018-10-20

  

  To confirm iPS identity and purity, we checked embryonic stem cell makers, Tra-1-81 (Fig. 2A) and Tra-1-60 (not shown) by live staining and found all concentration equation are Tra-1-81 positive. Acknowledgment The project described was supported by Award Number I21BX002215 (WX) from the Biomed

• The occurrence of airway epithelial thickening in asthma lea

  2018-10-20

  

  The occurrence of airway epithelial thickening in insulin pathway leads to a decrease in airway lumen size, consequently resulting in increased airway resistance corresponding to AHR (Elias, Zhu, Chupp, and Homer, 1999). Data from pediatric and non-fatal asthma studies have shown epithelial thickne

• Creating a genetically diverse set of differentiated

  2018-10-20

  

  Creating a genetically diverse set of differentiated 5-ht3 for the purposes of phenotype-genotype studies would have great value in pharmacogenomics, a field that has relied primarily on EBV-transformed lymphoblastoid cell lines (Moen et al., 2012; Wheeler and Dolan, 2012; Welsh et al., 2009). Tissu

• br Resource table Resource details

  2018-10-20

  

  Resource table: Resource details Expanded T calculate concentration isolated from a 38year-old Japanese healthy male were reprogrammed employing Sendai virus vectors (SeVdp) expressing four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4. SeVdp is integration-free vector, and the absence of rep

• br Materials and methods br Acknowledgments We would like to

  2018-10-20

  

  Materials and methods Acknowledgments We would like to thank Dr. Saran Karumbayaram for providing guidance in the culture and expansion of the primate fibroblasts. We would also like to thank Steven Dowdy for depositing the T7-VEE-OKS-iG plasmid (Addgene). This project was funded by NIH grant

• miR a has been extensively studied

  2018-10-20

  

  miR-29a has been extensively studied in different fields. miR-29a has been reported to be associated with cardiac hypertrophy and fibrosis (Roncarati et al., 2014), and functions as a negative regulator of extracellular matrix remodeling (van Rooij et al., 2008). We have previously reported that miR

• These results with fresh non fractionated

  2018-10-20

  

  These results with fresh, non-fractionated, not culture-expanded, adipose tissue-derived SVF are comparable with other two previously reported clinical series, using cultured adipose-derived ADSCs with controlled cell doses. The adipose tissue contains large populations of mesenchymal caffeic acid p

• Although the present study focuses predominantly on keratino

  2018-10-20

  

  Although the present study focuses predominantly on keratinocytes, it has been known for many years that fibroblasts play an important role in wound healing and in remodeling the extracellular matrix. Thus, an ideal bioengineered graft will always need to incorporate fibroblasts as well as keratinoc

• Further support for the hierarchical two

  2018-10-20

  

  Further support for the hierarchical two-stem-cell model came with the discovery of an additional reserve ISC marker locus, Hopx, encoding an atypical homeodomain protein with functions in early heart development (Chen et al., 2002). An IRES-CreERT2 cassette inserted into the endogenous Hopx locus r

• Cell cycle adaptations in human ESCs

  2018-10-20

  

  Cell-cycle adaptations in human ESCs (hESCs) are less defined. In contrast to mESCs, hESCs exhibit significant fluctuation of cyclin expression in a cell-cycle-dependent manner (Neganova et al., 2009), indicating differences in the regulation of key cell-cycle proteins between the two cell types. Si

• A role for PI K controlling ENPs

  2018-10-20

  

  A role for PI3K controlling ENPs fits well with current ideas of how FGF, BMP, β-catenin, and FAT4 regulate ENP self-renewal and differentiation (Figure 4C). The ENPs all receive the differentiation promoting β-catenin signal from the ureteric bud, but only the TAK-242 that receive a stromal signal

• br Experimental Procedures Extended details outlining specif

  2018-10-20

  

  Experimental Procedures Extended details outlining specific steps and protocols can be found in the Supplemental Experimental Procedures. Author Contributions Acknowledgments Introduction Proximal spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder caused by t

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